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2 edition of Regulation of gene expression in Lactococcus lactis. found in the catalog.

Regulation of gene expression in Lactococcus lactis.

Tracy Jayne Eaton

Regulation of gene expression in Lactococcus lactis.

by Tracy Jayne Eaton

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Published by University of East Anglia in Norwich .
Written in English


Edition Notes

Thesis (Ph.D.), University of East Anglia, School of Biological Sciences, 1993.

ID Numbers
Open LibraryOL21847187M

@article{osti_, title = {The ltp gene of temperate Streptococcus thermophilus phage TP-J34 confers superinfection exclusion to Streptococcus thermophilus and Lactococcus lactis}, author = {Xingmin, Sun and Goehler, Andre and Heller, Knut J and Neve, Horst}, abstractNote = {The ltp gene, located within the lysogeny module of temperate Streptococcus thermophilus phage TP-J34, has been. The expression vector's job begins when it enters the host cell because it is used for the primary purpose to expression of gene(s). Cloning vectors Very similar to expression vectors, involve the same process of introducing a new gene into a plasmid, but the plasmid is then 5/5(2).

CodY is a nutritional regulator mainly involved in amino acid metabolism. It has been extensively studied in Bacillis subtilis and Lactococcus lactis. We investigated the role of CodY in gene regulation and virulence of the human pathogen Streptococcus pneumoniae. We constructed a codY-mutant and examined the effect on gene and protein expression by microarray and 2D DIGE analysis. Lactic acid bacteria (LAB) comprise different groups of microorganisms, such as Carnobacterium, Enterococcus, Tetragenococcus, Vagococcus, Weissella as well as species of genera which constitute the “industrial” core of LAB, like Lactococcus, Lactobacillus, Streptococcus, Pediococcus and Leuconostoc [].LAB reside in different natural habitats, including healthy and decaying plants, milk Cited by: 5.

The strain Lactococcus lactis NZ The strain of bacteria we chose, Lactococcus lactis NZ, is created by integrating the nisK and nisR genes for the signal transduction system into the pepN gene (broad range amino peptidase) of L. lactis subsp. cremoris MG (nisin-negative). When a gene of interest is subsequently placed behind the inducible promoter PnisA in the plasmid pNZ, the.   Mierau I, Kleerebezem M: 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis. Appl Microbiol Biotechnol. , /s CAS.


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Regulation of gene expression in Lactococcus lactis by Tracy Jayne Eaton Download PDF EPUB FB2

Abstract. Undoubtedly, the second-best genetically characterized Gram-positive bacterium is Lactococcus lactis, following behind the unchallenged model system Bacillus gh there are quite a number of similarities between these two organisms with respect to gene regulation and gene expression in general, it is important to realize there are also important : Oscar P.

Kuipers, Kees J. Leenhouts. Lactococcus lactis is a Gram-positive bacterium used extensively in the production of buttermilk and cheese, but has also become famous as the first genetically modified organism to be used alive for the treatment of human disease.

lactis cells are cocci that group in pairs and short chains, and, depending on growth conditions, appear ovoid with a typical length of - : Bacilli. LmrCD is a major MDR transporter in Lactococcus lactis: Chapter 3.

LmrR is a transcriptional repressor of the expression of the Multidrug ABC transporter LmrCD in Lactococcus lactis: Chapter 4. Distinct mechanisms of LmrR-mediated gene: regulation of multidrug resistance in. Multidrug resistance (MDR) in Lactococcus lactis is due to the expression of the membrane ATP-binding cassette (ABC) transporter LmrCD.

In the absence of drugs, the transcriptional regulator LmrR. Detailed knowledge of sugar metabolism and the regulators controlling gene expression in Lactococcus lactis may contribute to the improvement of mechanisms controlling significant cellular processes in these bacteria.

In the case of industrial microorganisms, acting on the defined regulatory network may drastically affect the properties of the Cited by: 9. Marugg JD, Meijer WC, Vankranenburg R, Laverman P, Bruinenberg PG and de Vos WM () Medium-dependent regulation of proteinase gene expression in Lactococcus lactis: Control of transcription initiation by specific dipeptides.

Bacteriol. (11): – PubMed Google ScholarCited by: Sang-Ryoung Kim, Kyung-Min Yeon, in Comprehensive Analytical Chemistry, Two-Component Regulatory System.

Inthe autoregulation system for Gram-positive bacteria was first described in Lactococcus Regulation of gene expression in Lactococcus lactis. book (Kuipers et al., ).This report found that the expression level of gusA is directly related to a number of signaling molecules added extracellularly.

Find many great new & used options and get the best deals for The Lactic Acid Bacteria: Genetics of Lactic Acid Bacteria 3 (, Hardcover) at the best online prices at.

In L. lactis the osmoregulation of glycine betaine uptake is modulated by transport activity and the gene expression of the transporters OpuA and BusR that act as osmoregulator and osmosensor. First, deletion of the CIRCE element located upstream of the monocistronic dnaJ gene of Lactococcus lactis resulted in a strong constitutive expression.

Second, site-directed mutagenesis of the sequence within CIRCE in the dnaK operon of B. subtilis resulted in a higher level of dnaK expression at 37° C. Third, the distance between the groE Cited by: G.

Stanley, in Encyclopedia of Food Sciences and Nutrition (Second Edition), Mesophilic Starters. Lactococcus lactis (Lc. lactis) is the dominant acidifying mesophile species used, with Leuconostoc mesenteroides ssp. cremoris, a weakly acidifying culture, being used to impart flavor in certain (fresh) dairy fermentation.

lactis has two subspecies, namely cremoris and lactis, and also. This study describes the genetic analysis of the riboflavin (vitamin B 2) biosynthetic (rib) operon in the lactic acid bacterium Lactococcus lactis subsp.

cremoris strain NZ Functional analysis of the genes of the L. lactis rib operon was performed by using complementation studies, as well as by deletion analysis. In addition, gene-specific genetic engineering was used to examine which Cited by:   Characterization of the multi-gene AbiR phage defense system from Lactococcus lactis ssp.

lactis biovar. diacetylactis KR2. IFT annual meeting, Abstracts book. Progress 01/01/02 to 12/31/02 Outputs Nisin is a protein substance produced by certain strains of Lactococcus lactis, which are common constituents of cheese cultures.

Genetics of Lactic Acid Bacteria. Editors: Wood, Brian J.B., Warner, Philip J. (Eds.) will find this book invaluable. In addition, professors teaching under- and post-graduates in microbiology, and postgraduate research students will also find this an essential reference work.

Regulation of Gene Expression in Lactococcus lactis Pages. Aminoacyl-tRNA synthetases and translation factors are key enzymes required for pro tein biosynthesis. Escherichia coli and Bacillus subtilis often use different strategies to regulate the expression of the genes encoding these enzymes.

Synthesis of several E. coli aminoacyl-tRNA synthetases is controlled by different mechanisms acting at the transcriptional or translational by: 5. This scope of this chapter is limited to discussions of Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, and Streptococcus thermophilus, and summarizes and abstracts the major areas of research of lactic acid bacteria.

The historical association of the lactic acid bacteria with milk fermentation has encouraged a substantial amount of research on the biochemistry and genetics of lactose Cited by: 1 Introduction: Background, Relevant Genetic Techniques, and Terms Plasmid Biology, Conjugation, and Transposition Comparative Genomics of Bacteriophage Infecting Lactic Acid Bacteria Genetics of the Metabolism of Lactose and Other Sugars Regulation of Gene Expression in Lactococcus lactis Gene Expression in Lactobacilli Regulation of gene.

expression in Bacteria and their Viruses. Model of Lac repressor binding to Lac operator Todays lecture () Gene regulation Discovery of the lac system: negative control (evidence for operator and repressor as an example) Catabolite repression of the lac operon: positive control Dual positive and negative control: the arabinose operon Metabolic pathways and additional.

CodY of Lactococcus lactis MG is a transcriptional regulator that represses the expression of several genes encoding proteins of the proteolytic system. DNA microarray analysis, comparing the expression profiles of L. lactis MG and an isogenic strain in which codY was mutated, was used to determine the CodY regulon.

In peptide-rich medium and exponentially growing cells. This study describes the genetic analysis of the riboflavin (vitamin B2) biosynthetic (rib) operon in the lactic acid bacterium Lactococcus lactis subsp.

cremoris strain NZ Functional analysis of the genes of the L. lactis rib operon was performed by using complementation studies, as well as by deletion analysis. In addition, gene-specific genetic engineering was used to examine which Cited by:.

Vanda Mimi Palmer (VMP), an orchid hybrid of Vanda tesselata and Vanda Tan Chay Yan is a highly scented tropical orchid which blooms all year round.

Previous studies revealed that VMP produces a variety of isoprenoid volatiles during daylight. Isoprenoids are well known to contribute significantly to the scent of most fragrant plants. They are a large group of secondary metabolites which may Cited by: The importance of balanced expression of glycolytic genes in Lactococcus lactis Materials and methods The experimental procedures employed are described briefly here and will be de-scribed in more detail elsewhere [4].

Lactococcus lactis subsp. cremoris MG was used as a model organism for modulating the expression of the las genes.The most widely used protein expression system for L.

lactis is the NICE (Nisin Controlled Gene Expression) system in which the expression of the gene of interest is tightly regulated, and high expression levels are achieved using nisin, a food-grade molecule, as an : Rodolfo Garza-Morales, Beatriz E.

Rendon, Mohammad Tariq Malik, Jeannete E. Garza-Cabrales, Anne Auc.